As populations around the world continue to age, there is increasing interest in finding natural compounds that can promote healthy aging and reduce the risk of age-related diseases. In this study, we investigated whether clove extract could promote healthy aging using yeast, a widely accepted model organism for studying the biology of aging. We found that treatment with clove extract significantly increased the lifespan of aging yeast cells. The extract reduced intracellular ROS levels, decreased oxidative damage to proteins, and improved the ability of the organism to survive under stressful conditions. Importantly, clove extract also reduced the formation of protein aggregates and stimulated autophagy, a natural cellular recycling process that removes damaged proteins and cellular components. These findings suggest that clove extract helps maintain proteostasis, or protein quality control, which is essential for preserving cellular health and function during aging.

Further chemical analysis identified eugenol as the primary substance responsible for these anti-aging effects. The study also showed that these effects depend on the Ras/PKA signaling pathway, indicating that eugenol influences cellular mechanisms that regulate aging rather than acting solely as an antioxidant. Overall, this research demonstrates that clove extract and eugenol can promote healthier aging in yeast by reducing cellular damage and improving protein maintenance systems. These findings provide valuable insights into the potential use of natural plant-derived compounds to support healthy aging and prevent age-related cellular decline.