Lect. Dr. Titipatima Sakulterdkiat, M.D
Email: titipatima.sak@mahidol.ac.th
Tel: 662-201-5572
EDUCATION
2014 – 2019: Doctor of Medicine, Ramathibodi Hospital, Mahidol University Bangkok, THAILAND
2007 – 2013: Ph.D in Pathobiology Mahidol University
1. Faculty of Graduate Studies (Salaya campus) Nakhonpathom, THAILAND
2. Faculty of Sciences (Phyathai campus) Bangkok, THAILAND
2003 – 2007: Bachelor’s in Biological Sciences, majoring in Molecular Cell Biology, California State University, San Marcos, CA 92096, USA
OCCUPATION
2013 – present: Lecturer
Faculty of Science, Mahidol University, Bangkok, THAILAND
AWARDS/ SCHOLARSHIPS
• June 2008 – 2013: Royal Golden Jubilee Ph.D. Program Scholarship (THAILAND)
• June 2008: Institutional Strengthening Program Scholarship, Faculty of Science, Mahidol University 2008 (THAILAND) (accepted)
• June 2004 – May 2007: Research Initiative for Scientific Enhancement (RISE) Undergraduate Scholar, Office for Biomedical Research and Training (OBRT) (USA)
• November 2005: ABRCMS poster presentation award, Molecular Biological Sciences: Genomics section* (USA)
• 2003, 2007: Dean’s List (CSUSM, USA)
CERTIFICATION
2018 – 2020: Certified Ramathibodi Hospital CPR Training Center Advanced Cardiovascular Life Support (ACLS) Program
2007: Online certified non VA version, Institutional Animal Care and Use Committee (IACUC)
PROFESSIONAL ASSOCIATION
• 2019: Member of Medical Council of Thailand
• 2013: Member, Protein Society of Thailand (PST)
• 2010 – 2012: Student member, Protein Society of Thailand (PST)
• 2004 – 2007: Student member, American Society for Microbiology (ASM)
EXPERIENCE
Mahidol University, Faculty of Medicine (December 2015)
➤ Medical student leader in Community Medicine course
• Organize and plan research project along with group of 20 medical students in field survey of inappropriate pharmaceutical drug use in Sawanpraya, Khonburi, Nakhon Ratchasima province, Thailand
Mahidol University, Faculty of Sciences (November 2007 – 2013)
➤ Graduate Researcher
• Chulabhorn Research Institute (2009-2013):
• Effect of hypoxia on expression of stem cell characteristics in human liver cancer cells
• Study curcumin resistance induced by hypoxia in HepG2 cells
• Mahidol University Campus (2009):
• Cultured various cell lines: human ovarian adenocarcinoma cell lines wildtype and cisplatin resistant: A2780/A2780CP and 2008/2008CP, mouse fibroblast L929
➤ Teacher’s Assistant (2009 – 2011)
• TA Pathobiology section in Medical courses for third year MD students
Bangkok Oncoserv (October 2007)
➤ Graduate researcher
• Observed/Learned tumor cell isolation from tissue biopsy for in vitro drug sensitivity testing to determine most effective drug or drug combinations for therapy
Stanford University (October 2006)
➤ Invited scholar to participate in the 1st Stanford Biosciences Preview weekend
California State University, San Marcos (CSUSM)
➤ Biotech Club Treasurer (September 2006 – May 2007)
• Learn about Student life and Leadership skills
➤ Supplemental Instruction Leader (January 2006 – May 2007)
• Head focus group discussions on Biostatistics: Experimental Design and Statistical Analysis
➤ Undergraduate Researcher (October 2004 – May 2007)
• Analysis of gene expression of a gamma and delta class Carbonic Anhydrase in eukaryotic marine alga, Emiliania huxleyi, using qRT-PCR
• Identification of genes and proteins involved in C4 Photosynthesis in Emiliania huxleyi
• Cloning and heterologous expression of Malic enzyme from Emiliania huxleyi
PUBLICATIONS
- Sakulterdkiat, C. Srisomsap, R. Udomsangpetch, J. Svasti and K. Lirdprapamongkol. Curcumin Resistance Induced by Hypoxia in HepG2 Cells is Mediated by Multidrug-resistance-associated Proteins. Anticancer Research 32:5337-5342 (2012). Impact Factor 2012: 1.713. 5-Year Impact Factor: 1.775.
- Doungchawee, D. Sutdan, K. Niwatayakul, T. Inwisai, A. Sitthipunya, N. Boonsathorn, T. Sakulterdkiat, W. Sirawaraporn and V. Thongboonkerd. Development and evaluation of an immunochromatographic assay to detect serum anti-leptospiral lipopolysaccharide IgM in acute leptospirosis. Scientific Reports 7, Article number: 2309 (2017). 5-Year Impact Factor: 4.847.
CONFERENCE PRESENTATIONS
- Titipatima Sakulterdkiat, Chantragan Srisomsap, Rachanee Udomsangpetch, Jisnuson Svasti, and Kriengsak Lirdprapamongkol. Curcumin Resistance Induced by Hypoxia in HepG2 Cells is Mediated by Multidrug Resistance-associated Proteins. Poster Presentation, The 7th Princess Chulabhorn International Science Congress (PC) Cancer: From Basic Research to Cure (November 29 – December 3, 2012) Bangkok, THAILAND
- Titipatima Sakulterdkiat, Daranee Chokchaichamnankit, Kriengsak Lirdprapamongkol, Rachanee Udomsangpetch, Jisnuson Svasti and Chantragan Srisomsap. Proteomic Analysis of Hypoxiainduced Curcumin Resistance in HepG2 Human Liver Cancer Cell Line. Poster Presentation, 2nd International Congress on Analytical Proteomics (ICAP) (July 18-20, 2011) Ourense, SPAIN.
- Titipatima Sakulterdkiat, Kriengsak Lirdprapamongkol, Rachanee Udomsangpetch, Jisnuson Svasti and Chantragan Srisomsap. Investigation of Mechanisms Involved in Hypoxia-induced Curcumin Resistance in HepG2 Human Liver Cancer Cell Line. Poster Presentation, The 3rd Biochemistry and Molecular Biology (BMB) International Conference: “From Basic to Translational Research for a Better Life” (April 6-8, 2011) Chiang Mai, THAILAND.
- Titipatima Sakulterdkiat, Kriengsak Lirdprapamongkol, Rachanee Udomsangpetch, Jisnuson Svasti and Chantragan Srisomsap. Multidrug Resistance Proteins (MRPs) Contribute to Hypoxiainduced Curcumin Resistance in HepG2 Human Liver Cancer Cell Line. Poster Presentation, RGJ-Ph.D Congress XII (April 1-3, 2011) Pattaya, Chonburi, THAILAND.
- Titipatima Sakulterdkiat, Kriengsak Lirdprapamongkol, Rachanee Udomsangpetch, Jisnuson Svasti and Chantragan Srisomsap. Oxygen and Curcumin Resistance in Liver Cancer. Oral Presentation, Mahidol University, Faculty of Science (MUSC) Graduate Research Exposition 2010 (October 26-27, 2010) Bangkok, THAILAND. *SC STAR (Faculty of Science’s Students with Talent and Achievement in Research).
- Titipatima Sakulterdkiat, Kriengsak Lirdprapamongkol, Rachanee Udomsangpetch, Jisnuson Svasti and Chantragan Srisomsap. Effects of Hypoxia on Drug Resistance in HepG2 Human Liver Cancer Cell Line. Poster Presentation, The 5th Annual Symposium of Protein Society of Thailand (PST) (June 23-25, 2010) Bangkok, THAILAND.
- Titipatima Sakulterdkiat, Thomas Wahlund, Ph.D., Tammy Tran, Betsy Read, Ed.D. Cloning and Heterologous Expression of a Malic Enzyme and Alanine Aminotransferase cDNA from the Unicellular Calcifying Alga, Emiliania huxleyi. Poster Presentation, Annual Biomedical Research Conference for Minority Students (ABRCMS) (November 8-11, 2006) Anaheim, CA, USA.
- Zheng, S. Sakulterdkiat, N. Whisenhand, D. Olszova, B.A. Read, T.M. Wahlund. Initial Studies on the Expression and Regulation of delta-Carbonic Anhydrase and its Role during Carbon Acquisition and Metabolism in Emiliania huxleyi. Poster Presentation, American Society for Microbiology (ASM) Annual Meeting (May 2006) Orlando, FL, USA.
- Titipatima Sakulterdkiat, Thomas Wahlund, Ph.D. Expression and Initial Characterization of Two Genetically-Distinct Carbonic Anhydrases from the Marine Alga, Emiliania huxleyi, Under Carbon and Zinc Limitation Conditions. Oral Presentation, Statewide CSU Student Research Competition (May 2006) Camarillo, CA, USA.
- Titipatima Sakulterdkiat, Thomas Wahlund, Ph.D., Betsy Read, Ed.D., Hong Zheng, BA., Sarah Meagor. The Expression and Initial Characterization of delta-Carbonic Anhydrase from the Marine Alga, Emiliania huxleyi, Under Carbon and Zinc Limitation Conditions. Poster Presentation, Annual Biomedical Research Conference for Minority Students (ABRCMS) (November 2005) Atlanta, GA, USA. *Conference Award.
- Sarah Meagor, Thomas Wahlund, Ph.D., Betsy Read, Ed.D., Hong Zheng, Titipatima Sakulterdkiat. The Effects of Zinc and Bicarbonate Limitation on Gene Expression of delta-Carbonic Anhydrase in the Marine Alga, Emiliana huxleyi. Poster Presentation, Annual Biomedical Research Conference for Minority Students (ABRCMS) (November 2005) Atlanta, GA, USA.
TECHNICAL SKILLS
Molecular Biology
• RNA/DNA extraction
• cDNA synthesis
• PCR and qRT-PCR analysis (gene expression analysis)
• Primer design and sequence analysis
• Transformation and Molecular Cloning
• siRNA transient gene knockout
Proteomics
• Protein extraction
• Western blot
• Zymogram
• 2-dimensional gel electrophoresis
• Protein in-gel digestion for LC/MS/MS analysis
Cell Biology
• Cell culture: eukaryotic marine algae, bacteria and human cell lines
• Migration and Invasion assays
• Tube formation assay
• MTT and Crystal violet assays
• Hollow Fiber culture system (mimic in vivo environment)
Computational Analysis
• iCycler, Roche LightCycler and Amersham Biosciences qRT-PCR software analysis
• Prism 15, Minitab 14, and SPSS statistical software analysis
EXTRACURRICULAR ACTIVITIES
• Master of Ceremony at SC STAR Mahidol University, Faculty of Science (MUSC) Graduate Research Exposition (October 30, 2012) Bangkok,
• Assistant Vice President of the Pathobiology graduate student council (2009 – 2010) Bangkok, THAILAND
• English Tutor (2008-2009) Bangkok, THAILAND
– Preparations for MU-TOEFL Exam with emphasis on Listening Comprehension and Structure/Grammar sections
– Led group discussions on English speaking skills
• Private Tutor for Biology, Mathematics and General Chemistry (September 2006 – May 2007, February – March 2010) CA, USA.